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Direct probing: covalent attachment of probe DNA to double-stranded target DNA.

We report a novel procedure, which can be applied to probing of specific DNA, for covalently attaching probe DNA to complementary sequences in double-stranded target DNA. Employing hairpin-like oligonucleotide probes in combination with successive use of recA protein and DNA ligase, probes can be at...

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Bibliografiske detaljer
Main Authors: Fujiwara, J, Oishi, M
Format: Artigo
Sprog:Inglês
Udgivet: 1998
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC148029/
https://ncbi.nlm.nih.gov/pubmed/9838005
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