Improvement of fragment and primer selection for mutation detection by denaturing gradient gel electrophoresis.

Denaturing gradient gel electrophoresis (DGGE) is one of the most powerful methods for mutation detection currently available. For successful application the appropriate selection of PCR fragments and PCR primers is crucial. The sequence of interest should always be within the domain with the lowest...

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Detalhes bibliográficos
Main Authors: Wu, Y, Hayes, V M, Osinga, J, Mulder, I M, Looman, M W, Buys, C H, Hofstra, R M
Formato: Artigo
Idioma:Inglês
Publicado em: 1998
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC148013/
https://ncbi.nlm.nih.gov/pubmed/9826769
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