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Improvement of fragment and primer selection for mutation detection by denaturing gradient gel electrophoresis.

Denaturing gradient gel electrophoresis (DGGE) is one of the most powerful methods for mutation detection currently available. For successful application the appropriate selection of PCR fragments and PCR primers is crucial. The sequence of interest should always be within the domain with the lowest...

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Published in:Nucleic Acids Res
Main Authors: Wu, Y, Hayes, V M, Osinga, J, Mulder, I M, Looman, M W, Buys, C H, Hofstra, R M
Format: Artigo
Language:Inglês
Published: Oxford University Press 1998
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148013/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9826769/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.23.5432
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