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Yeast artificial chromosome segregation from host chromosomes with similar lengths.

We propose a new method for segregation of yeast artificial chromosomes (YACs) from endogenous yeast chromosomes with similar lengths. The method is based on recently developed PNA-assisted rare cleavage (PARC) of genomic DNA. We apply the PARC procedure to YAC-containing samples of yeast DNA in suc...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Izvolsky, K I, Demidov, V V, Bukanov, N O, Frank-Kamenetskii, M D
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 1998
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147924/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9776769/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.21.5011
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