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Yeast artificial chromosome segregation from host chromosomes with similar lengths.

We propose a new method for segregation of yeast artificial chromosomes (YACs) from endogenous yeast chromosomes with similar lengths. The method is based on recently developed PNA-assisted rare cleavage (PARC) of genomic DNA. We apply the PARC procedure to YAC-containing samples of yeast DNA in suc...

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Detalles Bibliográficos
Main Authors: Izvolsky, K I, Demidov, V V, Bukanov, N O, Frank-Kamenetskii, M D
Formato: Artigo
Idioma:Inglês
Publicado: 1998
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC147924/
https://ncbi.nlm.nih.gov/pubmed/9776769
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