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Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy
We report here the design, synthesis and application of pyrene binary oligonucleotide probes for selective detection of cellular mRNA. The detection strategy is based on the formation of a fluorescent excimer when two pyrene groups are brought into close proximity upon hybridization of the probes wi...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2006
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1477857/ https://ncbi.nlm.nih.gov/pubmed/16769776 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl406 |
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