Altering the DNA-binding specificity of Mu transposase in vitro.
We describe the isolation of a variant of Mu transposase (MuA protein) which can recognize altered att sites at the ends of Mu DNA. No prior knowledge of the structure of the DNA binding domain or its mode of interaction with att DNA was necessary to obtain this variant. Protein secondary structure...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147725/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9671813/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.15.3521 |
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