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Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism.

PcrA from Bacillus stearothermophilus is a DNA helicase for which, despite the availability of a crystal structure, there is very little biochemical information. We show that the enzyme has a broad nucleotide specificity, even being able to hydrolyse ethenonucleotides, and is able to couple the hydr...

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Main Authors: Bird, L E, Brannigan, J A, Subramanya, H S, Wigley, D B
Formato: Artigo
Idioma:Inglês
Publicado: 1998
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC147586/
https://ncbi.nlm.nih.gov/pubmed/9592155
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