Preparation of active tRNA gene transcripts devoid of 3'-extended products and dimers.
Significant amounts (10-30%) of 3'-extended products with one or two extra nucleotides are synthesized in the course of run-off tRNA gene transcription with T7 RNA polymerase. Denaturing polyacrylamide gel electrophoresis appeared to be insufficient to provide preparative amounts of pure correct-siz...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
1998
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147566/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9580706/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.10.2500 |
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