Preparation of active tRNA gene transcripts devoid of 3'-extended products and dimers.
Significant amounts (10-30%) of 3'-extended products with one or two extra nucleotides are synthesized in the course of run-off tRNA gene transcription with T7 RNA polymerase. Denaturing polyacrylamide gel electrophoresis appeared to be insufficient to provide preparative amounts of pure correct-siz...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1998
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147566/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9580706/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.10.2500 |
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