Preparation of active tRNA gene transcripts devoid of 3'-extended products and dimers.

Significant amounts (10-30%) of 3'-extended products with one or two extra nucleotides are synthesized in the course of run-off tRNA gene transcription with T7 RNA polymerase. Denaturing polyacrylamide gel electrophoresis appeared to be insufficient to provide preparative amounts of pure correct-siz...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Kholod, N, Vassilenko, K, Shlyapnikov, M, Ksenzenko, V, Kisselev, L
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1998
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147566/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9580706/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.10.2500
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