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Efficient directional cloning of recombinant adenovirus vectors using DNA-protein complex.

We describe an efficient cloning system utilizing adenoviral DNA-protein complexes which allows the directional cloning of genes into adenoviral expression vectors in a single step. DNA-protein complexes derived from a recombinant adenovirus (AVC2.null) were isolated by sequential use of CsCl step g...

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Bibliographic Details
Main Authors: Okada, T, Ramsey, W J, Munir, J, Wildner, O, Blaese, R M
Format: Artigo
Language:Inglês
Published: 1998
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC147483/
https://ncbi.nlm.nih.gov/pubmed/9518487
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