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Mutagenesis of Neisseria meningitidis by In Vitro Transposition of Himar1 mariner

Now that the meningococcal genome sequence has been completed, the lack of a suitable method for saturation mutagenesis remains a major obstacle to the unraveling of the pathogenic propensity of Neisseria meningitidis. Here, we demonstrate that in vitro Himar1 mariner transposition on chromosomal or...

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Detalhes bibliográficos
Main Authors: Pelicic, Vladimir, Morelle, Sandrine, Lampe, David, Nassif, Xavier
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC110981/
https://ncbi.nlm.nih.gov/pubmed/10986241
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