Nalaganje...
An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes.
The QuikChangeTM protocol is one of the simplest and fastest methods for site-directed mutagenesis, but introduces mutations at only one site at a time, and requires two HPLC-purified complementary oligonucleotides. Here, we describe that this method can be used with non-overlapping oligonucleotides...
Shranjeno v:
| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1998
|
| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC147442/ https://ncbi.nlm.nih.gov/pubmed/9512562 |
| Oznake: |
Označite
Brez oznak, prvi označite!
|