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An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes.

The QuikChangeTM protocol is one of the simplest and fastest methods for site-directed mutagenesis, but introduces mutations at only one site at a time, and requires two HPLC-purified complementary oligonucleotides. Here, we describe that this method can be used with non-overlapping oligonucleotides...

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Bibliografiska uppgifter
Huvudupphovsmän: Kirsch, R D, Joly, E
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1998
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC147442/
https://ncbi.nlm.nih.gov/pubmed/9512562
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