A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli.

The high affinity binding interaction of biotin to avidin or streptavidin has been used widely in biochemistry and molecular biology, often in sensitive protein detection or protein capture applications. However, in vitro chemical techniques for protein biotinylation are not always successful, with...

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Bibliographische Detailangaben
Hauptverfasser: Smith, P A, Tripp, B C, DiBlasio-Smith, E A, Lu, Z, LaVallie, E R, McCoy, J M
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1998
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC147411/
https://ncbi.nlm.nih.gov/pubmed/9490786
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