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Optimization of elastolysis conditions and elastolytic kinetic analysis with elastase from Bacillus licheniformis ZJUEL31410

The solubilization of elastin by Bacillus licheniformis elastase cannot be analyzed by conventional kinetic methods because the biologically relevant substrate is insoluble and the concentration of enzyme-substrate complex has no physical meaning. In this paper we report the optimization of elastoly...

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Auteurs principaux: Chen, Qi-he, He, Guo-qing
Format: Artigo
Langue:Inglês
Publié: Zhejiang University Press 2006
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1473995/
https://ncbi.nlm.nih.gov/pubmed/16691643
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1631/jzus.2006.B0482
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