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Optimization of elastolysis conditions and elastolytic kinetic analysis with elastase from Bacillus licheniformis ZJUEL31410

The solubilization of elastin by Bacillus licheniformis elastase cannot be analyzed by conventional kinetic methods because the biologically relevant substrate is insoluble and the concentration of enzyme-substrate complex has no physical meaning. In this paper we report the optimization of elastoly...

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Detalhes bibliográficos
Main Authors: Chen, Qi-he, He, Guo-qing
Formato: Artigo
Idioma:Inglês
Publicado em: Zhejiang University Press 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1473995/
https://ncbi.nlm.nih.gov/pubmed/16691643
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1631/jzus.2006.B0482
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