Large CAG/CTG repeat templates produced by PCR, usefulness for the DIRECT method of cloning genes with CAG/CTG repeat expansions.
We report here a simple method for generating large CAG/CTG repeat sequences. We have applied this method to clone the genomic sequence containing the CAG/CTG repeat and its upstream intronic sequence present in spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) by a modified DIRECT...
שמור ב:
| הוצא לאור ב: | Nucleic Acids Res |
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| Principais autores: | , , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
1998
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147394/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9469848/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.5.1352 |
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