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Large CAG/CTG repeat templates produced by PCR, usefulness for the DIRECT method of cloning genes with CAG/CTG repeat expansions.

We report here a simple method for generating large CAG/CTG repeat sequences. We have applied this method to clone the genomic sequence containing the CAG/CTG repeat and its upstream intronic sequence present in spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) by a modified DIRECT...

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Sonraí Bibleagrafaíochta
Main Authors: Pujana, M A, Volpini, V, Estivill, X
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1998
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC147394/
https://ncbi.nlm.nih.gov/pubmed/9469848
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