Large CAG/CTG repeat templates produced by PCR, usefulness for the DIRECT method of cloning genes with CAG/CTG repeat expansions.

We report here a simple method for generating large CAG/CTG repeat sequences. We have applied this method to clone the genomic sequence containing the CAG/CTG repeat and its upstream intronic sequence present in spinocerebellar ataxia type 3 or Machado-Joseph disease (SCA3/MJD) by a modified DIRECT...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Pujana, M A, Volpini, V, Estivill, X
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1998
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147394/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9469848/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.5.1352
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