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Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR.

A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this m...

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Bibliografiske detaljer
Main Authors: Bonnet, S, Prévot, G, Bourgouin, C
Format: Artigo
Sprog:Inglês
Udgivet: 1998
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC147339/
https://ncbi.nlm.nih.gov/pubmed/9461480
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