Binding of double-stranded DNA by Escherichia coli RecA protein monitored by a fluorescent dye displacement assay.

We have developed a new assay to characterize the double-stranded DNA (dsDNA) binding properties of RecA protein. This assay is based on measurement of changes in the fluorescence of a 4',6-diamidino-2-phenylindole (DAPI)-dsDNA complex upon RecA protein binding. The binding of RecA protein to a comp...

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Cyhoeddwyd yn:	Nucleic Acids Res
Prif Awduron:	Zaitsev, E N, Kowalczykowski, S C
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Oxford University Press 1998
Pynciau:	Research Article
Mynediad Ar-lein:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147265/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9421529/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.2.650
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