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Binding of double-stranded DNA by Escherichia coli RecA protein monitored by a fluorescent dye displacement assay.

We have developed a new assay to characterize the double-stranded DNA (dsDNA) binding properties of RecA protein. This assay is based on measurement of changes in the fluorescence of a 4',6-diamidino-2-phenylindole (DAPI)-dsDNA complex upon RecA protein binding. The binding of RecA protein to a...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Zaitsev, E N, Kowalczykowski, S C
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1998
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC147265/
https://ncbi.nlm.nih.gov/pubmed/9421529
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