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High-Molecular-Weight Protein (pUL48) of Human Cytomegalovirus Is a Competent Deubiquitinating Protease: Mutant Viruses Altered in Its Active-Site Cysteine or Histidine Are Viable

We show here that the high-molecular-weight protein (HMWP or pUL48; 253 kDa) of human cytomegalovirus (HCMV) is a functionally competent deubiquitinating protease (DUB). By using a suicide substrate probe specific for ubiquitin-binding cysteine proteases (DUB probe) to screen lysates of HCMV-infecte...

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Autors principals: Wang, Jianlei, Loveland, Amy N., Kattenhorn, Lisa M., Ploegh, Hidde L., Gibson, Wade
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1472576/
https://ncbi.nlm.nih.gov/pubmed/16731939
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00401-06
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