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High-Throughput Transposon Mutagenesis of Corynebacterium glutamicum and Construction of a Single-Gene Disruptant Mutant Library

A simple and high-throughput transposon-mediated mutagenesis system employing two different types of transposons in combination with direct genomic DNA amplification and thermal asymmetric interlaced PCR (TAIL-PCR) was developed. Each of the two minitransposons based on IS31831 (ISL3 family) and Tn5...

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Sonraí Bibleagrafaíochta
Main Authors: Suzuki, Nobuaki, Okai, Naoko, Nonaka, Hiroshi, Tsuge, Yota, Inui, Masayuki, Yukawa, Hideaki
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 2006
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1472376/
https://ncbi.nlm.nih.gov/pubmed/16672528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.5.3750-3755.2006
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