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New Multiple-Deletion Method for the Corynebacterium glutamicum Genome, Using a Mutant lox Sequence
Due to the difficulty of multiple deletions using the Cre/loxP system, a simple, markerless multiple-deletion method based on a Cre/mutant lox system combining a right-element (RE) mutant lox site with a left-element (LE) mutant lox site was employed for large-scale genome rearrangements in Coryneba...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1317446/ https://ncbi.nlm.nih.gov/pubmed/16332837 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8472-8480.2005 |
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