New Multiple-Deletion Method for the Corynebacterium glutamicum Genome, Using a Mutant lox Sequence

Due to the difficulty of multiple deletions using the Cre/loxP system, a simple, markerless multiple-deletion method based on a Cre/mutant lox system combining a right-element (RE) mutant lox site with a left-element (LE) mutant lox site was employed for large-scale genome rearrangements in Coryneba...

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Bibliographic Details
Main Authors: Suzuki, Nobuaki, Nonaka, Hiroshi, Tsuge, Yota, Inui, Masayuki, Yukawa, Hideaki
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2005
Subjects:
Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317446/
https://ncbi.nlm.nih.gov/pubmed/16332837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8472-8480.2005
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