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Single substitutions of phosphorothioates in the HDV ribozyme G73 define regions necessary for optimal self-cleaving activity.

Phosphorothioate (NTPalphaS) analogues were incorporated into the HDV genomic ribozyme by transcription with T7 polymerase. The introduction of a sulfur in place of the pro-Rp oxygen at the phosphate 5'to positions A64, A63, A43, U27, G62, C61, C44, C41, C22and C21appeared to inhibit self-cleav...

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Bibliografiska uppgifter
Huvudupphovsmän: Prabhu, N S, Dinter-Gottlieb, G, Gottlieb, P A
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1997
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC147158/
https://ncbi.nlm.nih.gov/pubmed/9396824
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