Isolation of nuclei for chromatin analysis in fission yeast.
The methods available for analysis of the chromatin of Schizosaccharomyces pombe are time consuming (>8 h) and/or result in some degradation of the chromatin. Here we report an optimised method for the preparation of spheroplasts and the isolation of nuclei which takes <25 min and is suitable for an...
Spremljeno u:
| Izdano u: | Nucleic Acids Res |
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| Glavni autori: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1997
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147094/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9358188/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.22.4700 |
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