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Isolation of nuclei for chromatin analysis in fission yeast.

The methods available for analysis of the chromatin of Schizosaccharomyces pombe are time consuming (>8 h) and/or result in some degradation of the chromatin. Here we report an optimised method for the preparation of spheroplasts and the isolation of nuclei which takes <25 min and is suitable for an...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nucleic Acids Res
Egile Nagusiak: Mason, J A, Mellor, J
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 1997
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147094/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9358188/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.22.4700
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