Interaction of the phage T4 Dam DNA-[N6-adenine] methyltransferase with oligonucleotides containing native or modified (defective) recognition sites.

The DNA-[N 6-adenine]-methyltransferase (Dam MTase) of phage T4 catalyzes methyl group transfer from S-adenosyl-l-methionine (AdoMet) to the N6-position of adenine in the palindromic sequence, GATC. We have used a gel shift assay to monitor complex formation between T4 Dam and various synthetic dupl...

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Detalles Bibliográficos
Main Authors: Malygin, E G, Petrov, N A, Gorbunov, Y A, Kossykh, V G, Hattman, S
Formato: Artigo
Idioma:Inglês
Publicado: 1997
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC147042/
https://ncbi.nlm.nih.gov/pubmed/9336474
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