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Detection and isolation of RNA-binding proteins by RNA-ligand screening of a cDNA expression library.

A screening assay for the detection of RNA-binding proteins was developed. It allows the rapid isolation of cDNA clones coding for proteins with sequence-specific binding affinity to a target RNA. For developing the screening protocol, constituents of the human U1 snRNP were utilized as model system...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Principais autores: Sägesser, R, Martinez, E, Tsagris, M, Tabler, M
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1997
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146985/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9380503/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.19.3816
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