Detection and isolation of RNA-binding proteins by RNA-ligand screening of a cDNA expression library.
A screening assay for the detection of RNA-binding proteins was developed. It allows the rapid isolation of cDNA clones coding for proteins with sequence-specific binding affinity to a target RNA. For developing the screening protocol, constituents of the human U1 snRNP were utilized as model system...
Kaydedildi:
| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1997
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146985/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9380503/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.19.3816 |
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