Expression of bovine mitochondrial tRNASer GCU derivatives in Escherichia coli.
By replacing a stretch of five A-U base pairs in the acceptor stem with G-C pairs, mitochondrial tRNA-SerGCU lacking a D arm could be expressed in Escherichia coli cells in considerable amounts. The expressed tRNA with no modified nucleoside was serylated in vitro with the mitochondrial enzyme. The...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1997
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146909/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9254711/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.17.3503 |
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