Expression of bovine mitochondrial tRNASer GCU derivatives in Escherichia coli.

By replacing a stretch of five A-U base pairs in the acceptor stem with G-C pairs, mitochondrial tRNA-SerGCU lacking a D arm could be expressed in Escherichia coli cells in considerable amounts. The expressed tRNA with no modified nucleoside was serylated in vitro with the mitochondrial enzyme. The...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Hayashi, I, Kawai, G, Watanabe, K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146909/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9254711/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.17.3503
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