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The elimination of primer-dimer accumulation in PCR.

We attempted to produce primer-dimers (PDs) from a variety of primers with differing types and extents of complementarity. Where PDs were produced they were cloned and sequenced. We were unable to produce detectable PDs either with individual primers alone or with similar sequence primers even if th...

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Hlavní autoři: Brownie, J, Shawcross, S, Theaker, J, Whitcombe, D, Ferrie, R, Newton, C, Little, S
Médium: Artigo
Jazyk:Inglês
Vydáno: 1997
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC146890/
https://ncbi.nlm.nih.gov/pubmed/9241236
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