Magnetic bead capture of cDNAs from double-stranded plasmid cDNA libraries.
We have developed a cDNA library screening method which allows the simultaneous screening of >10 ( 12 ) double-stranded plasmid cDNA molecules with minimal a priori sequence knowledge. A biotinylated, gene-specific oligonucleotide probe along with abutting 'blocking' oligos is hybridized to the plas...
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| Vydáno v: | Nucleic Acids Res |
|---|---|
| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1997
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146847/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9224622/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.15.3183 |
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