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Magnetic bead capture of cDNAs from double-stranded plasmid cDNA libraries.

We have developed a cDNA library screening method which allows the simultaneous screening of >10 ( 12 ) double-stranded plasmid cDNA molecules with minimal a priori sequence knowledge. A biotinylated, gene-specific oligonucleotide probe along with abutting 'blocking' oligos is hybridized to the plas...

詳細記述

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書誌詳細
出版年:Nucleic Acids Res
主要な著者: Shepard, A R, Rae, J L
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1997
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146847/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9224622/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.15.3183
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