Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation.

We have developed a genetic means to recover sequences from YAC-ends near the yeast selectable marker URA3. This strategy is based on the ability of URA3 to complement mutations in pyrF, an Escherichia coli gene required for pyrimidine biosynthesis. We have developed an E.coli strain with a non-reve...

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Autors principals: Wright, D A, Park, S K, Wu, D, Phillips, G J, Rodermel, S R, Voytas, D F
Format: Artigo
Idioma:Inglês
Publicat: 1997
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Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC146780/
https://ncbi.nlm.nih.gov/pubmed/9185581
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