Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation.

We have developed a genetic means to recover sequences from YAC-ends near the yeast selectable marker URA3. This strategy is based on the ability of URA3 to complement mutations in pyrF, an Escherichia coli gene required for pyrimidine biosynthesis. We have developed an E.coli strain with a non-reve...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Wright, D A, Park, S K, Wu, D, Phillips, G J, Rodermel, S R, Voytas, D F
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1997
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146780/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9185581/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.13.2679
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