A closed tube format for amplification and detection of DNA based on energy transfer.

A new method for the direct detection of PCR-amplified DNA in a closed system is described. The method is based on the incorporation of energy transfer-labeled primers into the amplification product. The PCR primers contain hairpin structures on their 5'ends with donor and acceptor moieties located...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Nazarenko, I A, Bhatnagar, S K, Hohman, R J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146748/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9171107/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.12.2516
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!