A closed tube format for amplification and detection of DNA based on energy transfer.
A new method for the direct detection of PCR-amplified DNA in a closed system is described. The method is based on the incorporation of energy transfer-labeled primers into the amplification product. The PCR primers contain hairpin structures on their 5'ends with donor and acceptor moieties located...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146748/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9171107/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.12.2516 |
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