Pentapeptide scanning mutagenesis: random insertion of a variable five amino acid cassette in a target protein.
A new insertion method for probing protein functional organization was developed. The method relies on the random insertion of transposon Tn 4430 and subsequent in vitro deletion of the bulk of the transposon after which a 15 bp insertion remains within the target gene. This results in pentapeptide...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1997
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146650/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9108174/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.9.1866 |
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