Thermodynamic analysis of monoclonal antibody binding to duplex DNA.
A technique based on fluorescence polarization (anisotropy) was used to measure the binding of antibodies to DNA under a variety of conditions. Fluorescein-labeled duplexes of 20 bp in length were employed as the standard because they are stable even at low ionic strength yet sufficiently short so t...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1997
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146592/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9060442/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.7.1442 |
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