Optimization of DNA shuffling for high fidelity recombination.

A convenient 'DNA shuffling' protocol for random recombination of homologous genes in vitro with a very low rate of associated point mutagenesis (0.05%) is described. In addition, the mutagenesis rate can be controlled over a wide range by the inclusion of Mn2+or Mg2+during DNase I digestion, by cho...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Zhao, H, Arnold, F H
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146579/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9092645/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.6.1307
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