Optimization of DNA shuffling for high fidelity recombination.
A convenient 'DNA shuffling' protocol for random recombination of homologous genes in vitro with a very low rate of associated point mutagenesis (0.05%) is described. In addition, the mutagenesis rate can be controlled over a wide range by the inclusion of Mn2+or Mg2+during DNase I digestion, by cho...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146579/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9092645/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.6.1307 |
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