Sequencing double-stranded DNA by strand displacement.
Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1997
|
| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146469/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016613/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.3.677 |
| Tags: |
Keine Tags, Fügen Sie das erste Tag hinzu!
|