Sequencing double-stranded DNA by strand displacement.

Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of...

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izdano v:	Nucleic Acids Res
Principais autores:	Fu, D J, Köster, H, Smith, C L, Cantor, C R
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 1997
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146469/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016613/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.3.677
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