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Selective amplification of RNA utilizing the nucleotide analog dITP and Thermus thermophilus DNA polymerase.

The ability to selectively amplify RNA in the presence of genomic DNA of analogous sequence is cumbersome and requires implementation of critical controls for genes lacking introns. The convenient approaches of either designing oligonucleotide primers at the splice junction or differentiating the ta...

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Detalles Bibliográficos
Main Authors: Auer, T, Sninsky, J J, Gelfand, D H, Myers, T W
Formato: Artigo
Idioma:Inglês
Publicado: 1996
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC146350/
https://ncbi.nlm.nih.gov/pubmed/9016675
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