Selective amplification of RNA utilizing the nucleotide analog dITP and Thermus thermophilus DNA polymerase.

The ability to selectively amplify RNA in the presence of genomic DNA of analogous sequence is cumbersome and requires implementation of critical controls for genes lacking introns. The convenient approaches of either designing oligonucleotide primers at the splice junction or differentiating the ta...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Auer, T, Sninsky, J J, Gelfand, D H, Myers, T W
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 1996
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146350/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016675/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.24.5021
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