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Expression cloning of cDNA by phage display selection.

Expression cloning of a mouse kappa chain fragment has been achieved from a cDNA library by display of expressed proteins on filamentous phage and affinity selection for binding to anti-mouse Fab antibodies. Expressed proteins were anchored to the phage coat by a synthetic, anti-parallel leucine zip...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Light, J, Maki, R, Assa-Munt, N
Format: Artigo
Language:Inglês
Published: Oxford University Press 1996
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146248/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8932399/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.21.4367
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