Efficient random subcloning of DNA sheared in a recirculating point-sink flow system.

Based on a high-performance liquid chromatographic pump, we have built a device that allows recirculation of DNA through a 63-microm orifice with ensuing fractionation to a minimum fragment size of approximately 300 base pairs. Residence time of the DNA fragments in the converging flow created by a...

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Detalhes bibliográficos
Main Authors: Oefner, P J, Hunicke-Smith, S P, Chiang, L, Dietrich, F, Mulligan, J, Davis, R W
Formato: Artigo
Idioma:Inglês
Publicado em: 1996
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC146200/
https://ncbi.nlm.nih.gov/pubmed/8918787
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